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All five containers demonstrated high cell tolerance at concentrations of up to 1 mM in cell lines originating from kidney, liver or blood tissue using assays for metabolic activity and cytotoxicity.
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There was an active accumulation of Actin11 transcripts peaking at 3.25 mm in cells undergoing growth acceleration, followed by a negative peak of almost the same amplitude at 4.25 mm in cells just starting to decelerate their growth.
The existence of these complexes was not surprising given that trivalent As species have high affinity for thiols, and the thiol-containing GSH can be present at up to 10 mM in cells.
Stable transfected clones were selected using 0.01 mM hygromycin in cell medium.
KA (Sigma) was prepared as a concentrated stock solution (1 mM) in the cell culture medium.
After filtration through a nylon mesh, Ca2+ was gradually reintroduced up to 1.8 mM in the cell suspension.
Compound 7a exhibited a TI value of >13.2 with CC50 value of >0.787 mM in C8166 cells.
The intracellular NAD+ levels are typically between 0.2 and 0.5 mM in mammalian cells, and change during a number of physiological processes [54].
An ATP molecule is a multifunctional nucleotide that exists at a concentration of ∼3.2 mM in the cells [7].
We determined substrate concentrations below 1 mM in the cell-containing, aqueous phases after a reaction time of 30 min.
Cardol has also been shown to be cytotoxic at a dose of 0.01 mM in HeLa cells [ 62].
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