Sentence examples similar to mm icn from inspiring English sources
Kinase activity was determined with [γ-P]ATP (ICN) in a buffer containing 30 mM Tris, pH 7.6, 10 mM KCl, 0.5 mM DTT. Full-length c-Jun was used as substrate.
Although the ICN identification at the group level directly reflects reliable ICNs with excellent signal-to-noise ratio compared to single subject ICNs, single subject ICNs showed sufficient quality for further comprehensive analysis at single subject level.
For metabolic labeling, cells were washed once with PBS and then incubated for 30 min (prior to indicated timepoint) at 37°C with 1 ml Methionine-free DMEM (Sigma) that was supplemented with 2% FBS, 100 I.U./ml penicillin, 100 µg/ml streptomycin, 2 mM L-glutamine, 10 mM HEPES (pH 7.4), and 90 µCi 35S-TransLabel (ICN) per 2×106 cells.
Templates for splicing substrates were generated by digestion of pEZ_ISS/WT with Nar I. Labeled RNA was synthesized from linear plasmid in a 50 µl reaction containing 2 µg of DNA, 0.4 mM each of ATP and GTP, 0.1 mM UTP and CTP, 2.5 µl of [32P]-UTP and [32P]-CTP (ICN), 0.5 mM diguanosine triphosphate and 2 µl of 20 U/µl T7 RNA polymerase Plus (Ambion, Austin, TX).
Radioactively-labeled intron transcripts were synthesized in 50 µl reactions containing 1 µg of PCR generated template, 0.5 mM each of ATP and GTP, 0.1 mM UTP and CTP, 2.5 µl of [32P]-UTP and [32P]-CTP (ICN) and 2 µl of 200 U/µl T7 RNA polymerase Plus (Ambion).
