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In the current paper, a series of high velocity impact tests using ϕ50 and ϕ25 mm ice spheres and 0.32 g granite stones on non-crimp fabric (NCF) composite plates are reported.
Transfected cells were removed from flasks following incubation in 1 mM ice cold EDTA in PBS.
Briefly, a gold nanoparticle seed solution was prepared by adding 600 μl of 10 mM ice cold sodium borohydride to a 10 ml 250 μM gold chloride solution in 100 mM CTAB, under vigorous stirring.
Briefly, cells were removed from flasks in 1 mM ice cold EDTA in PBS and then incubated at 4°C for 1 hr with 5 μg/ml Fy6 monoclonal antibody to human DARC or with isotype control antibody.
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After that, a freshly prepared 0.6 mL of 10 mM ice-cold NaBH4 solution was quickly injected into the mixed solution and magnetically stirred for 2 min.
Briefly, a seed solution was prepared by mixing 5 mL of CTAB (0.2 M) and 5 mL of HAuCl4 0.5 mM) with 0.6 mL freshly prepared 10 mM ice-cold NaBH4solution.
Nuclei were pelleted and resuspended in 0.2 mM ice-cold EDTA, pH 7.0 for 1 hour (this hypotonic treatment forces nucleosomes into the supernatant).
Tissue sample was homogenized in 5 vol. of 50 mM ice-cold phosphate buffer (pH 7.5).
The phosphorylation was initiated with 7.5 μl 10 mM ice-cold ATP.
Then, 0.6 mL of an aqueous NaBH4 solution (10 mM, ice-cold) was added in one shot under vigorous stirring.
In brief, after different treatments, the H9C2 cells (cardiomyocytes line) were collected and homogenized in 50 mM ice-cold potassium phosphate buffer (pH 6.8).
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