Sentence examples for mm forward and from inspiring English sources
The belt was reduced in less critical areas, to 120 mm forward and 100 mm aft.
The ships were protected with an armored belt that was 150 mm thick amidships and reduced to 70 mm forward and 40 mm aft.
Each 25 µL reaction contained 12 µL SYBR Green, 0.1 µL 25 mM MgCl2, 0.25 µL each of 10 mM forward and reverse primer, 9.9 µL ddH2O (nuclease-free water, GrowCells) and 2 µL of cDNA.
The PCR consisted of 12.5 µl of SYBR Green PCR Master Mix, 1 µl of 10 mM forward and reverse primers, 10.5 µl water, and 1 µl template cDNA in a total volume of 25 µl.
For rtPCR reactions using TSG polymerase, each 50 µL reaction contained 0.4 µL polymerase, 1 µL 10 mM dNTPs, 5 µL 10X buffer, 3.5 µL 25 mM MgSO4, 1 µL each of 50 mM forward and reverse primer, 36.1 µL ddH2O (nuclease-free water, GrowCells) and 2 µL of cDNA.
The reaction mixture contained 10 ng genomic DNA, 10 μL of MeltDoctor™ HRM Master Mix Applied Biosystemss, California, USA), 0.2 μL of 10 mM forward and reverse primers.
The accuracy of the pupil tracker when the model eye (focused well at the center of the image) was moved by 5 mm forwards and backwards was 15 ± 4 μm [ 9].
The PCR reaction mixture consisted of 2 μl of cDNA template (150 ng/μl), 0.2 μl of 10-mM dNTP mix, 1 μl of 10-mM forward and reverse oligonucleotide primer, 0.2 μl of Taq DNA polymerase (1 unit), 2 μl of 10× ThermoPol reaction buffer, and 14.6 μl of ddH2O, in a total volume of 20 μl.
Each PCR was carried out in a 20 μL reaction volume composed of 2 μL cDNA template, 10 μL of SYBR Green PCR Master Mix Applied Biosystemss, Carlsbad, CA, USA), 0.5 mM forward primer, and 0.5 mM reverse primer.
All PCR reactions were performed in a 20-μl total volume, containing 0.5 U of Taq polimerase (Qiagen, Germany), deoxyribonucleotide triphosphate (dNTP) 0.4 mM, MgCl2 1.5 mM and forward and reverse primers, 200 nM each.
The microsatellite fragments were amplified by PCR containing 20 ng of template DNA, 2.5 μL of 1× PCR buffer (20 mM Tris HCl [pH 8.4] and 50 mM KCl), 2.0 μL (10.0 mM) of forward and reverse primer, 1.5 μL of dNTP mix (2.5 mM of each base), 1.5 μL of MgCl2 (1.5 mM), and 1 unit of Taq DNA polymerase (Fermentas, Thermo Fisher Scientific, Pittsburgh, Pennsylvania, USA).
