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Brushings were obtained from the peripheral airways using a 1.8 mm brush.
To isolate the SOX9+ BCs, 2 mm brush with samples were cut with scissors into 1 cm pieces.
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After routine anesthesia, a 2 mm disposable brush (Wiltek Medical, Winston-Salem, NC) was inserted into the working channel of the bronchoscope and advanced to the airways distal to the orifice of the desired lobar bronchus.
Approximately 20,000 30,000 cells were brushed off from the luminal surface of donor's 3rd 4th order bronchus using a 2-mm bronchoscopic brush (Wimberley et al., 1982) (Fig. 1B).
Our solution was first to entice a small spider on to the tip of a small soft paintbrush and then to touch the ramp with the tip of the brush 10 mm from ramp's upper end.
The mechanically wounded plants that were punched with a brush leaving 0.5 1 mm holes spaced 2 mm apart were used a positive control.
In fed-batch mode, the configuration with three larger brushes (25 mm diameter) produced 80% more power (1240 mW m−2) than reactors with eight smaller brushes (8 mm) (690 mW m−2).
The naturally developed purple MFC effluent was collected from a single-chamber air-cathode MFC (4.5 L) having four homemade brush anodes (60 mm in diameter and 150 mm in length) inoculated with the primary clarifier overflow of a local wastewater treatment plant.
Brass rings (Φ = 14 mm) (University of Manchester Medical School Engineering Workshop, Manchester, England) were brushed with a separation agent (petroleum jelly) and then filled with dental stone.
Preliminary experimental results show that the throughput of this method can be over 1000 mm2 minute with a brush of 100 mm diameter.
Results presented in Fig. 15 confirm that, at 100 mM NaCl, a fully functional brush layer is formed upon their adsorption on silica and polystyrene.
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