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The epicenter and adjacent sections were dissected into 1 mm blocks and fixed in 2% glutaraldehyde for 2 h before being washed three times in 0.1 M cacodylate buffer with 5.3 mM CaCl2, and then incubated with 1% osmium tetroxide (Electron Microscopy Sciences, Hatfield, PA, 19190) with 1.5% potassium ferrocyanide (BDH, Toronto, Canada) for 1.5 h.
Tissue to be embedded in Epon blocks was cut in approximately 2 × 2 × 2 mm blocks with a razorblade or scalpel and rinsed in 0.10 M phosphate buffer (pH 7.4) for 2 × 5 min, then dehydrated in graded ethanol solutions and embedded in Epon.
Following different protocols, the EHTs were divided into approximately 1 mm blocks and immediately fixed with cold 2% glutraldehyde.
The tissue was cryopreserved in 27% sucrose and the cords were sectioned into 1 mm blocks and embedded in OCT compound.
Briefly, placental chorionic villi were isolated, minced into 2 3 mm blocks, and washed extensively with RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum (FCS), 1% penicillin-streptomycin and 1% L-glutamine (washing medium).
Endometrium were cut into 1 2 mm blocks and placed on top of the collagen sponge gels at the interface between the medium and the air (3 blocks per collagen sponge and per well).
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The general antioxidant and glutathione analogue N-acetyl-L-cysteine (NAC, 5 mM) blocked MCT1 and CD147 overexpression in glucose-starved SiHa cells (Figure 4h).
Afterwards, the spinal cord was cut twice using scissors (once rostral to T10 and once caudal to T10) for complete transection, removing a 2 mm block of the spinal cord.
The 2 by 2 cm by 3 mm block of limestone was then mounted onto high-purity quartz round-mounts using standard geologic thin-sectioning adhesive resins.
Staying with the sodium alginate used in the present work, the contribution of MG and MM block cannot be overseen.
Cells were subsequently released for 9 hours in regular growth media prior to the second thymidine (2 mM) block.
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