Anti-acetyl-lysine, anti-MLC and anti-phospho(Ser19 -MLC were obtained from Cell Ser19 -MLCTechnology (Beverly, MA), anti-MLCK werefrobtained-Aldrich.
MLC-GFP cells were obtained from the Drosophila Genomics Resource Center.
Human glioblastoma multiforme tissue (frozen) and brain biopsy tissue from MLC patients (both frozen and paraffin-embedded) were obtained for diagnostic purposes and used for research purposes with informed consent of patients and families.
Transgenic mice and wild-type littermate mice were obtained from offspring of MLC-pro transgenic mice mating with B6SJL F1.
Swab specimens were obtained from women who underwent routine pelvic examinations at sexually transmitted disease clinics in 6 US cities. Cultured T. vaginalis isolates were tested for nitroimidazole resistance (aerobic minimum lethal concentration [MLC] >50 µg/mL).
Blood samples (anticoagulated with EDTA) were obtained from a live, stranded yearling female harbor porpoise (Phocoena phocoena) that required humane killing in northern North Carolina in May 2005 (MLC 001) and from a yearling male harbor porpoise.
Breeding pairs for CA-MLCK Tg mice and MLCKKO mice have been obtained from Dr. Turner's laboratory at University of Chicago (Su et al, 2009).
Molecular evidence of Bartonella infection was obtained by direct PCR on whole blood from MLC 001, and by using a recently improved diagnostic method that combines preenrichment blood culture (24 ) and real-time PCR in samples from AAH 009.
Further confirmation of the cardiac phenotype was obtained by investigating the relative expression levels of MLC-2v, a gene which displays absolute cardiac tissue specificity [ 40].
rga-3 feeding clone was obtained from Ahringer lab (Gurdon institute, Cambridge, United Kingdom), ect-2 and mlc-4 from Hyman lab (MPI-CBG, Dresden, Germany).
The potential benefits involved in the use of VMAT relative to standard IMRT are obtained with enhanced degrees of freedom in continuously modulating the multileaf collimator (MLC) field shape, gantry rotation speed and dose rate.
