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Plasma samples (0.1 ml) were weighed and the radioactivity was measured in a Wallac γ-counter.
Each sample of LF1 (0.5 mL) and SRS solution (0.5 mL) were weighed in 2 mL twist-top Eppendorf tubes, diluted with water (1 mL) and acidified (with 72% mass H2SO4) to a pH of 0.3.
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Each sample of LF1 (0.5 mL) was weighed in 1.5 mL Eppendorf tubes and diluted with water (0.5 mL).
Samples (15 mg) were weighed into 2 ml Eppendorf tubes and 1 ml sodium phosphate buffer (20 mM, pH 6.5) added.
Samples of CMV supplemented with rice straw (200 mg) were weighed into 100 ml calibrated glass syringes with pistons lubricated with Vaseline.
Dried cotton fibres (50 mg) were weighed into 2 mL screwcap tubes.
Samples (8 mL overlying water or 0.4 mL supernatant) were weighed into 40 mL clear glass vials with Teflon-lined septa (Brooks Rand) and diluted to 25 mL with 1% HCl.
Of homogenised wheat material, 105 ± 5 mg was weighed to 1.5-mL Eppendorf tubes and extracted with 1 mL of pre-cooled methanol/water 3 + 1 v/ v including 0.1 % formic acid, by vortexing for 10 s, and subsequent treatment in an ultrasonic bath at room temperature for 15 min according to de Vos et al. [ 19].
Following the pretreatment and rinse steps, the 250-mL collection bottles were weighed to determine the mass of fluid collected and the rinsate volume was quantitatively transferred to a 200-mL volumetric flask and brought to volume with deionized water.
The cell pellet and 0.1 mL of radioactive supernatant were weighed and assessed for radioactivity using a Packard 5500 gamma counter (Perkin-Elmer, CA, USA); the radioactivity concentration was expressed as cpm/g cells and cpm/mL medium, respectively.
Samples (8 mL) of filtered and whole overlying water were weighed into 40 mL vials.
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