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For cell wall removal, aliquots of 1 ml were treated with 250 KU/ml of lysozyme (Lyz; Novagen, Gibbstown, NJ) for 45 min at 37°C.
HL-60 cells (1×105 cells/ml, total 20 ml) were treated with 1.3% (v/v) DMSO and 2.5 µM retinoic acid.
PM1 cells (1.5×105/0.1 ml) and PBMCs (5×105/0.1 ml) were treated with SEVI with or without SP, and infected with HIV-1 BaL (200 pg p24/0.1 ml) for 2 h.
All specimens with the adjusted volume of 1.0 mL were treated with 1 mL of BioMérieux lysis buffer.
CLL cells (1 × 10 cells per ml) were treated both with/without 1 μ of IMD-0354 for 24 h.
Briefly, THP-1 cells in 12-well plates (10 cells/well, seeded in 1 ml) were treated as described above with 1 μM pigment.
Similar(43)
Fifty mL of cells (2.0 × 107 cells/mL) were treated with formaldehyde to cross-link proteins to DNA.
Plasma samples (0.20 ml) and the internal standard (Flunixin-d3; 40 ug (10 μL of 4.0 ng/ml)) were treated with 20 μL of 30% perchloric acid.
Briefly, HCT116 and KBM5 cells (5 × 10 cells/mL) were treated with 5 μM of indicated test sample in a final volume of 0.1 mL at 37°C for 72 h.
BMDM primed with LPS (500 ng/ml) were treated with quercetin, tamoxifen or serum-free medium.
Planktonic C. albicans (3.4 × 106 cfu/ml) were treated with C4 at the indicated concentrations for increasing time.
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