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EBC samples (almost 1 mL) were transported in dry ice to the laboratory and stored at –80°C in polypropylene tubes until analytical determinations.
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Sample-laden wipes were then placed in sterile 500 mL glass bottles, which were transported to the laboratory.
Plasma was decanted and replaced by 10 mL PBS and 1 mL 4% formalin and were transported, with constant rocking, to the Cytology Laboratory at the Cancer Center Karolinska (CCK) at the Karolinska Institute for analysis.
Samples from effusions were transported in 250 ml bottles containing 25 ml Dulbecco's modified Eagle's medium (DMEM; Sigma, Poole, UK) with antibiotics (100 IU ml−1 penicillin and 100 μg ml−1 streptomycin) and 1 M HEPES, but as a precaution to prevent blood clots forming while in transit, 5000 IU heparin sodium (Monoparin, CP Pharmaceuticals Ltd , Wales UK) was also added.
Samples were placed in 0.1 ml of sterile saline solution and were transported rapidly to the laboratory.
All the specimens were placed in 0.1 ml of sterile saline solution and were transported to the laboratory immediately and were stored at -70°C until further investigation.
Serum samples were kept in liquid nitrogen in 1-mL aliquots at Mekambo until they were transported to CIRMF.
Swabs were placed in 1.5-mL virus transport medium; all environmental samples were transported at 4°C within 36 hours to Institut Pasteur in Cambodia for subtype H5N1 testing by real-time reverse transcription PCR (rRT-PCR) after RNA extraction by using a viral RNA kit (QIAamp, QIAGEN, Valencia, CA, USA) and for virus isolation after inoculation onto MDCK cells.
Removed strings were transported to the laboratory within three hours in 15 ml Falcon tubes containing 1.5 ml 0.9% saline.
Samples were transported to the laboratory in culture media (10 ml) immediately following resection.
All tested ML bound with high affinity in a unique site, within the inner chamber of the protein, supporting that ML may be transported by Cel-Pgp-1.
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