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Aliquots (10 or 100 mL) were then transferred to Erlenmeyer flasks (25- or 250-mL flasks).
DI water (700 mL) and H2O2 (20 mL) were then added and stirred for 12 h.
Solutions of intermediates 2 (1.00 mmol) in acetonitrile (2 mL) were then added dropwise to the mixture of intermediates 1.
TEOS (51 g) and 37wt% HCl (140 mL) were then added into above aqueous solution to form a synthetic gel after 24-h stirring.
The cells (1 mL) were then harvested and resuspended in 10 mL of ice-cold 80% methanol and incubated for 1 h at room temperature.
Cells (0.5 mL) were then transferred to micro-tubes and treated with or without indoles and incubated for 3 h at 37 °C at 250 rpm.
Similar(25)
The number of colony forming units (cfu/ml) were then determined.
The indicated concentrations of antibodies, which varied from 0.01 ng/ml to 10 μg/ml, were then added.
Various amounts (10 100 μL) of sample solutions (10 mg/mL) were then separately taken into mini tubes.
The bacterial suspensions at 108 CFU/ml were then prepared by adjusting the optical density to OD620 nm = 0.110 ± 0.005 using a Jenway 6320D UV/visible light spectrophotometer.
The proteins (concentration ∼1 mg/mL) were then stored at −20°C.
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