Optimization of nitrogen sources: Medium with varying concentrations of Peptone and Yeast Extract (0.25, 0.5, 0.75, 1.0, 1.5 and 2.0 g/100 mL) was substituted in Sabourauds Dextrose Agar medium composition and the selected strain was inoculated for the optimized number of days.
The group 2 animals were designated as experimental and were treated similar to group 1 animals except the Ir.Cr (80 mg/kg) dissolved in normal saline (2 ml) was substituted for normal saline.
Then a mean of 1,364 mL (713 mL) of autologous blood was taken from the patients, and 1,459 mL (605 mL) of HAES 6% and 1,311 mL (742 mL) of crystalloid fluid were substituted as a local standard to account for preoperative deficits and guarantee a hematocrit below 0.25 during CPB.
Assays containing heptanal were performed like those described above except that 1.5 mL gastight vials were substituted for Eppendorf tubes.
However, it appears rather difficult to assess how much haemoglobin was functional when a blood loss of 1600 ml (containing approximately 800 ml HbY) was substituted with 1200 ml "healthy" PRBC with a haematocrit of 60%.
The supernatants were substituted by 1 ml of SB medium containing 100 μg ml−1 carbenicillin and 2.5 m M of isopropyl- β- D-thiogalactoside (IPTG, Sigma, St Louis, MO, USA); after that, the scFv induction was carried out for 18 h at 30 °C and 300 r.p.m.
Patients with 25OH-vitamin D <75 nmol/L [= 30 ng/mL] were substituted with 300000 IU cholecalciferol orally.
Blood loss caused by the blood volume remaining in the liver ranged from 300 to 700 ml and was substituted with donor erythrocytes and fresh frozen plasma units.
For the colourimetric control samples, 50 mL of distilled water was substituted for 50 mL of 1.5 mg mL−1 DTT.
For the blank, the 0.1 mL of 10% AlCl3 was substituted with distilled water.
Skeletal muscle K (SM-K) content was measured by flame photometry with an FLM 3 (Radiometer, Copenhagen, Denmark) with lithium as an internal standard using a solution as used for atomic absorption, except that 1.5 ml of redistilled water was substituted by 5 mmol l−1 LiCl.
