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Control samples of plasma (1 mL) were spiked with increasing quantities of SER to give a final drug concentration of 0.2-0.4 0.2-0.42 mL of acetone was added to 1.0 mL of the spiked plasma and was shaked gently.
Plasma samples (0.2 mL) were spiked with D3-β-AET as an internal standard (1 ng/mL), and steroids were extracted with 10 mL ethyl acetate, and evaporated under nitrogen.
Samples (1 ml) were spiked with 10 μl of oxibendazole (OBZ) (100 μg/ml), used as internal standard.
Briefly, the urine samples (1 mL) were spiked with an internal standard solution containing C4-MEHP, C4-MEOHP, C4-MEHHP, D4-MECPP, C4-MINP, and 4-MeUmb.
Plasma and abomasal fluid samples (1 mL) were spiked with 20 μL of OBZ (stock solution 50 μg/mL) as internal standard.
The urine samples (2 mL) were spiked with stable isotopically labeled TCPy, mixed well, and then subjected to an enzyme hydrolysis to liberate glucuronide- and sulfate-bound TCPy.
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Homogenized sample of 1 mL was spiked with 100 ng of the internal standard in the 4 mL headspace vial for quantitative analysis.
The turbot homogenate (10 ml) was spiked with E. tarda EIB202 to various original concentrations from 1 to 107 CFU/ml by tenfold serial dilutions.
The extract (1.8 mL) was spiked with 50, 100 and 150 µL (0.5, 1, 1.5 times the permitted level, respectively) of the mixed standard stock solution.
The plasma (0.5 mL) was spiked with 50 μL of IS working solution, after which it was vortex-mixed for 30 s and extracted with ethyl acetate (1 mL) by vortex mixing for 2 min.
Saliva including control samples or blank (bi-distilled water), 1.0 ml each, were spiked with [H]testosterone (Radiochemical Center, Amersham, UK, 1200 dpm/sample), and extracted in duplicate with diethyl ether (4 ml) in stoppered glass tubes.
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