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PNAC-1 cells (1 × 105 cells/mL, 0.5 mL) were seeded in 96-well plate and cultured for 24 h.
Briefly, 5 × 105 cells/5 ml were seeded in T-25 flask (Corning) and incubated overnight for better attachment.
DU-145 cells (10 ml) were seeded in a cell culture dish containing 2 × 106 cells and cultured overnight.
HL-60 cells (0.5 × 106 per ml) were seeded in T-25 tissue culture flasks and incubated with 1.5 mg dry roots equivalent/ml medium extract/ml.
HepG2 cells (0.5 × 10 cells per ml) were seeded in DMEM medium without antibiotics overnight.
The cells (2 × 10 cells per ml) were seeded into 96-well culture plates.
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SMMC-7221 liver carcinoma celiver2 mL, 5 × 10/mL) were seeded to 6-well plates and were carcinomaovernight.
Log-phase HCT116 cells (2 mL, 1 × 10 cells/mL) were seeded into 6-well culture plates.
Approximately 3.5 × 105 cells/ml were seeded per disk placed in each well.
The HT-29 cells at 2 × 105/mL were seeded in 24-well plates and cultured for 24 h.
MSCs (5 × 104 cells/ml) were seeded into 35-mm dishes and incubated for 12 h at 37 °C in 5% CO2.
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