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Samples (1.5 ml) were removed every 24 h and the presence of BLV proteins was determined using an indirect ELISA assay in which the antigen reaction with the monoclonal antibodies was evidenced by peroxidase labeled anti-mouse immunoglobulins.
Following the initial sampling, culture aliquots of 30 mL were removed at intermittent time points for the same purpose.
Samples of 2 ml were removed from the liquid cultivations at intervals and mycelium was separated from the supernatant by centrifugation.
At selected time intervals, aliquots (0.5 mL) were removed from the mixture solution, replacing by an equal volume (0.5 mL) of fresh SBF.
Thereafter, the 12 ml were removed and slides were gently rinsed twice with 12 ml of PPB to remove loosely attached cells.
During cultivation aliquots of 100 mL were removed from each of the cultures, at various time intervals, and were centrifuged at 6,800 × g for 15 min to separate the biomass from the supernatants.
Similar(26)
From the sample, 10 mL was removed.
A total of 1.2 mL was removed, from each tube, over the course of the experiment.
The release medium of 4 mL was removed and replaced by fresh buffer solution at regular time intervals.
At each time point 1.0 mL was removed from the 50-mL tube (outer bulk) and 1.0 mL of fresh buffer was added.
From the stock culture 0.2 ml was removed and dispersed in 20 ml of sterile phosphate buffer (50 mM, pH 7.0); vortex well.
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