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Thereafter, serially diluted samples (0.1 ml) were plated on nutrient agar medium (Oxoid) supplemented with 50 μg/ml nystatin to suppress the growth of fungi.
Enrichment broths (0.1 ml) were plated, after 2 and 5 days, on CCD agar (Oxoid, UK) and incubated microaerobically at 37°C [59].
Cells (5×104 cells/0.5 mL) were plated in the top chamber in FBS-free RPMI 1640 media and culture medium with 10% FBS RPMI 1640 was used in the bottom chamber as a chemo-attractant.
After 18 hrs, cells (15×104 cells/0.5 mL) were plated in the top chamber of a Matrigel-coated invasion plate (BD Bioscientific) and the invasion capacity of the cells was assessed as described above.
One million cells per ml were plated in dishes coated with 1 µg/ml anti-mouse CD3 antibody and 1 µg/ml anti-mouse CD28 antibody after labeling of T cells with 1 µm CarboxyFluorescein Succinimidyl Ester (CFSE).
Aliquots of 0.1 ml were plated on chocolate agar.
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One mL of nRBC or iRBC (107/mL) were plated in five replicates on a 24 well-plate and exposed to HBO (100% O2, 3.0 ATA) in a hyperbaric chamber for up to 6 hours.
For 3T3 cell transfection, 1.5×105 cells/mL were plated in 24-well plates in 0.5 mL DMEM+10% FBS.
PBMCs (5 × 105 cells/ml) were plated into 24-well plates (Nunc, Roskilde, Denmark) containing 2 ml of culture media and then cultivated in a 5% CO2/air mixture, humidified at 37°C.
Freshly isolated rat splenocytes (8 × 106 cells/mL) were plated in chamber slides (Lab-Tek) for 2 h to allow macrophages to adhere.
Isolated human PMNs (2.0 × 106 cells/mL) were plated on a 35-mm glass-bottomed dish (IWAKI, Tokyo, Japan), coated with 100 μL of poly-l-lysine solution (Sigma-Aldrich, St . Louis MO, USA), and incubated for 10 min.
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