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To perform two-step OptiPrep density gradient ultracentrifugation, 50% iodixanol (1 ml, Axis-Shield PoC AS, Oslo, Norway), 10% iodixanol (2 ml) and the sample (7 ml) were placed in an ultracentrifuge tube from bottom to top and then ultracentrifuged at 100,000 g for 2 h at 4 °C.
Freshly prepared SLN dispersions (2 ml) were placed in Amicon® Ultra-15 tubes.
Then, a few droplets of water (1.0 ml) were placed in those mixtures.
Stained cell suspensions (10 ml) were placed on a clean microscope slide and covered with a cover slip.
The polymer (0.1 g) and solvent (10 ml) were placed in an airtight vial and agitated about 25°C for 1 h.
As a typical synthetic procedure, 4 mL Se precursor and ODE (15 mL) were placed in a 100-mL round flask.
Similar(29)
Aliquots (1 mL) of cell suspension adjusted to 1 × 10 cells/mL were placed in 2-mL polypropylene tubes (Corning, New York, NY), which were then completely filled with culture medium.
Briefly, aliquots of 400 µl of PBMC suspended in complete culture media (5×106 cells/ml) were placed in 5 ml polypropylene tubes (BD, Heidelberg, Germany).
Two milliliters of methanol solution of DPPH radical in the concentration of 0.05 mg/mL and 1 mL of plant extract (1 mg/mL) were placed in cuvettes.
As started, 200 μL of NHS-activated MBs (1 mg/mL) were placed into a 1.5 mL microcentrifuge tube, and the supernatant was removed by a magnetic stand.
PBMCs (106 cells/ml) were placed in 24-well plates containing 1 ml αMEM with 10% fetal bovine serum (FBS), 100 units/ml penicillin, and 100 μg/ml streptomycin.
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