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GRN163L-treated myeloma cells (1 × 106 cells per ml) were mixed with annexin V-FITC and incubated in the dark for 15 min at room temperature (RT).
In this typical reaction, trans-stilbene (90.12 mg, 0.50 mmol), Fe2/mpg-C3N4, iron porphyrin, or Fe nanoparticle/mpg-C3N4 (ca. 3 nm) (0.50 µmol Fe) and DMAC (5 mL) were mixed in a 20 mL of Schlenk tube.
Briefly, an aqueous solution containing appropriate amount of ammonium paramolybdate (Sinopharm, (NH4 6Mo7O24·4H2O, 0.68 g) and chloroplatinic acid (Aldrich, H2PtCl6, 0.19 M, 2 mL) were mixed with the activated carbon (2 g) and then kept stirring for 12 h in the air at room temperature.
HCl (6.00 mL) were mixed into 450 mL water.
Plasma samples (1 ml) were mixed with 1 ml of 1 M acetic acid, then centrifuged.
Afterward, MCM-41 (1 g) and pyrrole (1 mL) were mixed in the filtered solution.
Similar(23)
One milligram of PEGylated MoS2 nanosheets and 0.1 mL PEI (50 mg/mL) were mixed in 2 mL deionized water.
In short, 1 ml of the extract and its derived fractions (1 mg/ml) were mixed with 1 ml of Folin-Ciocalteu's reagent.
Different concentration of extracts (31.25 500 µg/ml) were mixed with 1 ml of phosphate buffer (0.2 M, pH 6.6) and 1 ml of potassium ferricyanide (1%).
For DPPH activity measurement, extract (0.1 mg/mL) were mixed with 1 mL of 90 µM DPPH solution and then, final volume was made to 4 mL by adding 95% methanol.
Different concentrations of HMSNs (1, 5 and 10 mg/mL) were mixed with 1 mL of 1 mg/mL FITC in acetone solution.
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