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Actively growing starter cultures (100 mL) were inoculated into the fermenter, containing 5 L of autoclaved media.
Each of the vegetable sample suspensions (10 ml) were inoculated (by means of dipping) with 100 μl of bacterial suspension resulting in the initial load of ~105 cfu/g (Fatema et al. 2013; Feroz et al. 2013).
The BCG strain of Japan (1 × 107 colony-forming unit (CFU); Japan BCG Laboratory, Tokyo, Japan) was suspended in 0.2 ml of phosphate-buffered saline [15], or allogenic rat glioma cells (C6, 2 × 106 cells/0.2 ml) were inoculated into the left and right calf muscles of the animals to generate a rat model bearing both granulomas and tumours.
To prepare the bioformulations, X. stockiae PB09 seed culture (10 ml) were inoculated into LB broth (1000 ml) and placed in an incubator shaker at 200 rpm (New Brunswick Scientific, USA) and 28 °C for 48 h in the dark to obtain the bacteria at the concentration of approximately 1010 cells/ml which was used in the next step for producing the bioformulations as described in Table 1.
Then, 4 mL of brucellae suspension were added to 4 mL of blood, and these 8 mL were inoculated into BACTEC Plus + Aerobic/F bottles (Becton Dickinson, NJ, USA), and incubated at 37°C in a BACTEC 9240 device (Becton Dickinson, NJ, USA), until they were reported as positive.
The seed cultures (300 mL) were inoculated into the fermenter for batch cultivation.
Similar(32)
Each starter culture (0.1 mL) was inoculated into 5 mL of BT-2mM PCA medium.
10 ml sample was inoculated in double strength MacConkey broth media and rest 1 and 0.1 ml was inoculated in single strength MacConkey broth media.
A culture volume of 300 mL was inoculated with YNB-xylose freshly grown cells to a starting OD620 of 0.05.
The seed culture (10 mL) was inoculated into 100 × 500 mL Erlenmeyer flasks, each containing 100 g of wheat immersed in waterand sterilized.
An AR80 cell suspension (5 mL) was inoculated into each bottle containing culture medium and the bottles were incubated at 50 °C.
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