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Following the addition of internal standard (20 ng of 5, 6 dimethylbenzimidazole) plasma samples (0.75 ml) were extracted using a 3cc Waters Oasis MAX solid phase extraction cartridge.
Plasma samples (0.2 mL) were extracted using a Waters Oasis MAX (10 mg) 96-well plate and the resulting samples were analyzed using an Applied Biosystems API-5000 HPLC-MS/MS with an electrospray ionization (ESI) source.
Aliquots (2 mL) were extracted at desired time intervals, and another 2 mL fresh PBS was added to the system.
Regions of interest were positioned in superimposed CT and PET so as to use complementary visual information, and five (cylindrical) VOIRM (diameter 1.9 cm, height 2 cm) of a total volume of 30 mL were extracted per ldCT scan.
The protoplast extract (15 ml) were extracted from the cells (OD600 = 0.6, cultured in 100 mRCM containing 0.4% glycine), following a protocol developed by Mermelstein [22].
All breast milk samples (1 mL) were extracted in duplicates.
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The culture filtrate (500 ml) was extracted two times with ethyl acetate as solvent.
A total soil gas volume of 16 ml was extracted per sampling time.
Acetone was removed under vacuum, and the resulting aqueous layer (800 mL) was extracted three times with an equal volume of EtOAc to yield mycelial extract (13 g).
In the given study, a mean total blood volume of 1.8 ± 0.2 ml was extracted from each animal by manual sampling.
From this, a sample of approximately 0.1 mL is extracted, heparinized, converted to plasma and analyzed by a mid-infrared spectrometer.
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