The number of liposomes per ml was estimated by assuming there were 80,000 phospholipid molecules per liposome of 100 nm or 320,000 phospholipid molecules per liposome of 200-nm diameter [35].
Therefore, the surface nitrogen and the adsorbed CO2 densities (ML) are estimated by dividing the normalized intensities of the N 1s and O 1s XPS spectra by one third of that of the C 1s spectrum, respectively.
The recurrence of ML is estimated to occur within the first 5 years after clinical cure in approximately 15 50% of cases, even after successful antimonial therapy 17, 18.
Parasitaemia was evaluated using Giemsa-stained smears, which were prepared daily using blood taken from the tail veins, and the total number of parasitized erythrocytes per mL was estimated by counting in a Neubauer chamber.
First, at the time of primary tumor resection, median colony number 11 per 40 μl blood sample (Fig. 1b, bottom), thus the number of CTCs in whole blood (~1 ml) was estimated ~275.
However, due to the limited effects observed, only shallow slopes (0.00751 ms pg−1 ml and 0.0173 beats min−1 pg−1 ml, respectively) were estimated from the modelling.
ML analyses using RAxML with constrained topology were conducted and ML scores were estimated using the GTR+I+Γ model of sequence evolution.
This iteration was continued until the last two topologies converged (the final ML topology was almost always equal to the one in which the ML parameters were estimated from the parsimony topology).
ML parameters were estimated from an initial neighbor-joining tree and optimized in the construction of ML trees using variable base frequencies, a symmetrical substitution matrix and gamma distributed rate variation among sites.
Maximum Likelihood (ML) trees were estimated in PAUP*4.0b10 [41] using GTR + gamma models with parameters estimated from the data.
