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For harvesting mycelia, samples of 60 ml were drawn after 24 h and 30 h.
Samples (2 mL) were drawn at 4, 8, 24, 48, and 72 h to monitor the fermentation.
Blood samples (1.5 to 2.5 mL) were drawn at 5, 30, and 60 min following tracer administration for analysis of tracer metabolites in plasma.
The suspension was centrifuged at 4000 rpm for 10 min and after 10 min of settling, 2 ml were drawn from organic phase.
Thereafter, arterial blood samples (2 mL) were drawn manually at the midpoint of each frame until the end of measurement [13].
Blood samples (4 mL) were drawn at 2.5, 9, 20, 30, 40, 60, 90, 120, and 150 min for blood and plasma radioactivity and metabolite correction.
A median of 10 (range 8 to 10) venous blood samples (approx 5 ml) were drawn at nominal times of 5, 15, 30, 60, and 120 min and on multiple occasions up to 7 days following administration.
Aerosol samples (5 ± 1 mL) were drawn in long glass tube directly from the aerosol container and degassed by sonication for 5 min. 2 μL of degassed aerosol sample was spotted on TLC sheet in triplicate.
Blood samples (1.0 to 2.5 mL) were drawn at 4, 15, 30, 60, 90, and 120 min after the radioligand injection for blood and plasma radioactivity and metabolite correction.
A median of 10 (range, 9 to 10) venous blood samples (approximately 5 ml) were drawn at nominal times of 5, 15, 30, and 60 min after administration of 124I-IAZGP, and before and after each scan up to the nominal 24-h time point.
Similar(1)
Coverslips were placed in a parallel plate flow chamber maintained at 37°C and HL60 leukocytes (1.5×106 cells/mL) were drawn for 2 minutes across the monolayers at a flow rate of 0.26 mL/min, corresponding to at a shear stress of 0.5 dynes/cm2.
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