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For the cultivation in 96-microwell plates, aliquots (0.2 ml) were distributed in the microwells.
Six cylindrical VOIs (212 mL) were distributed in the main compartment on each SPECT/CT acquisition (Fig. 2).
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The CSF volume is approximately 150 ml in adults; 125 ml is distributed in the cranial and spinal subarachnoid spaces and 25 ml is found in the ventricles [1].
The cell suspension (1 mL) was distributed in a 24-well tissue culture plate (Corning) and cultured in the presence of APTX (5 µg/mL), Concanavalin A (Sigma) (2 µg/mL), or medium alone, at 37°C, in a humidified atmosphere with 5% CO2 for 72 h.
The filling-clearing procedure was repeated three or four times, until a target of 2.7 mL was distributed within the intertrabecular space.
The two MLs are distributed beneath the main roads in this district.
The 12-mL treatments were distributed 1 mL per well to 12 wells of a deep-well plate and incubated in the Duetz System.
Each strain and a water control (1.9 ml each) were distributed in 12 wells of a 2-ml 96-well deep-well plate (Greiner Bio-One, Alphen a/d Rijn, The Netherlands), of which 6 wells contained 100 μl of dH2O and 6 wells contained 100 μl of a 20% CaCl2 stock solution.
Samples were diluted to 1 ng/ul in 10 mM Tris, 1 mM EDTA pH 7.4, and 1 ml aliquots were distributed in plates of 64 samples (8 rows by 8 columns) before pooling.
To purify schizonts, 100 ml culture suspensions were distributed among three 50 ml tubes.
For adherent growth, 1 × 10 cells in 2 ml growth media were distributed in 6-well plates (Costar, tissue culture treated).
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