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Samples of the reaction mixture (0.1 ml) were diluted with 10 ml mixture of H2O CH3CN (1:1) and the absorbance of the solution was measured spectrophotometrically The effects of various parameters, such as time of reaction and type of solvent as well as the temperature of the reaction were studied in order to examine their effect on the reaction product pattern.
Aliquots of this supernatant (18 ml) were diluted by the addition of 34.5 ml of deionised water then treated with either 1.5 g of anion exchange resin (Dowex Marathon WBA), cation exchange resin (Dowex 50wx4-400) or activated carbon (Sigma-Aldrich plant cell culture tested C6289).
Briefly, sera (0.4 mL) were diluted 1∶5 in binding buffer, passed 3 times over 2 mL of packed resin and washed with 15 column volumes of binding buffer.
overnight at 37 °C and 2 ml were diluted in 200 ml of supplemented SB medium.
Peripheral blood samples (20 mL) were diluted by adding the same volume of PBS.
The methanolic shellfish extracts (1.2 mL) were diluted with 2.8 mL water.
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MBs (0.2 mL of 108 MBs/mL) were diluted with 0.3 mL of isotonic saline in 2-mL Eppendorf tubes (Eppendorf AG, Hamburg, Germany).
3D tumor spheroids formation was performed using the 3D Culture Matrix Basement Membrane Extract Reduced Growth Factor (Trevigen, Gaithersburg, MD, USA). 1 × 10 cells/ml were diluted in 24 ml of assay medium (98 ml of growth media plus 2 ml of 3D Culture Matrix RGF BME).
Briefly, 10 μL of plant extracts of known concentrations (20 mg/mL) were diluted with 0.5 mL of double distilled water.
The insoluble and soluble melanin concentrations (0, 1, 2, 4, 8, 16, 32, and 64 μg/mL) were diluted in 5 mL seawater in separate tubes and incubated at 28°C.
Plasma HIV-1 RNA levels >750,000 copies/ml were diluted and further assessed by dilution.
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