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Prior to imaging, three samples of ASCs (1.5 × 105 to 2 × 105 cells in 5 ml) were cultivated in a complete medium containing 20 μg/ml SPIO nanoparticles for 48 h.
In both cases, 10 cells/2 ml were cultivated in Mammocult Basal medium and 10% proliferation supplement (StemCell Technologies), supplemented with 0.2% heparin, and 10−3 M hydrocortisone on nonadherent plates at 37°C, 5% CO2.
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In order to show the effect of the antibiotic on the bacteria inside the eggs, bacteria in streptomycin (0.5 mg/mL) treated eggs (5 eggs were grinded and diluted in 1 mL sterile water) were cultivated on the Luria Bertani culture medium, and the bacteria in normal eggs were cultivated as control.
Whole blood was irradiated at 37 degrees C. Aliquots (0.2 ml) of whole blood were cultivated by the standard method.
Callus culture of the plant was derived from young leaves which were cultivated in 300 mL jars, containing 25 mL of Murashige and Skoog (MS) media [28], each supplemented with 2% sucrose, 0.5 mg/L 2,4-diphenoxy acetic acid (2,4-D), 2.5 mg/L naphthalene acetic acid anhydride (NAA), 0.01 g/L ascorbic acid and solidified by 0.6% agar at 25 ± 1°C in the dark.
Frozen stocks (−80°C) were cultivated in 100 mL YNBM in 1000 mL shake flasks at 30°C and 230 rpm.
The untreated original culture and the mutants were cultivated in 250 mL flasks containing 100 mL 9 K liquid medium with ferrous iron, and the initial density of cells was adjusted to about 1.0 × 10 cells/mL.
In this heterotrophic cultivation using various organic or inorganic compounds as a carbon source, the cultures were cultivated in 250 ml flasks containing 100 ml of each tested medium, with three replicates.
For liquid cultures, 20 to 30 agar blocks (1 mm) that were removed from the solid cultures were inoculated into flasks that contained 100 mL of malt media and were cultivated at 28°C for 7 days, under constant agitation at 150 rpm.
To study the properties of the xylanase activity production, the isolates having high xylanase activities were cultivated in 250 ml shake-flasks containing 50 ml basic xylanase production medium at 37 °C.
Yeast isolates were cultivated in 250 ml Erlenmeyer flasks containing 50 ml of mineral medium (MM).
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