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In brief, a small gastric pouch (15 ml) was created with the bowel in an antecolic and antegastric position.

A training dataset for ML was created out of twenty-three known targets of CREB transcriptional regulation [ 38, 39], and twenty-three genes out of a set of twenty-eight (Table 7) whose transcription regulations are potentially CREB-independent.

Due to the stacking fault formation, thin shell layers below 3 MLs are created, in which the compression mechanism mediates a further RS to ZB phase transition with reduced influence on the PL.

For RNA extraction, cultures of Ac. hypogyna and T. clavata were acclimatized at 25 °C for 2 weeks at 12 12 Light:Dark before 16 LB 50% concentration and 16 diH2O 150 ml subcultures were created, each with three autoclaved hemp seeds.

Two phylogenetic trees including a neighbor-joining (NJ) tree and a maximum-likelihood (ML) tree were created based on SNPs from whole genome sequences of the 7 clinical M. tuberculosis isolates and other 7 completely sequenced M. tuberculosis strains.

PNG renderings of the SBGN-ML files were created using PathVisio [ 90].

After the ileum was flushed with 5-10 ml of PBS, 8-10 loops were created by ligation using double ties and a 1-cm interval was left between the loops.

Initially, 250 mL of 8 M NaOH and 250 mL of 0.4 M MnCl2 solutions were created using anoxic DI water and nitrogen gas was used to purge the solutions.

Percoll density gradient columns were created in 250 ml graduated cylinders as previously described [ 42].

Agarose plugs for pulsed-field gel electrophoresis (PFGE) were created using 400 ml of log-phase culture following the method described in Eschbach et al. (1991) for three approximate cell counts of 5 × 10, 1 × 10, or 5 × 10 per plug.

Culture media with bacterial concentration of 105 colony-forming units (CFU /ml were created for each strain.

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