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The chambers were assembled using freshly prepared matrigel-coated filters and DMEM containing 0.8 mL NIH-3T3 as a chemoattractant in the lower compartment.
Desmin samples at a concentration of 0.2 to 0.5 mg/ml were assembled at 37°C for up to one hour after addition of an equal volume of 2 mM phosphate buffer (pH 7,5), 200 mM KCl.
Vimentin, or K5/K14 samples, in their respective dialysis buffer, at a concentration of 0.2 mg/ml were assembled at 37°C for up to one hour after addition of an equal volume of 40 mM Tris-HCl (pH 7) containing different concentrations of either NaCl and CaCl2.
Images were assembled using Photoshop.
Chromatograms were assembled in DNASTAR Lasergene SeqMan Pro v.7.1.0.
Reactions were assembled identical to those in gel-shift assays, except that RNasin (Promega) was included at a final concentration of 1 U/ml.
Four transcriptomes were assembled.
92,511 scaffolds were assembled.
The microfluidic chambers were assembled and coated with PORN (50 µg/ml)/laminin (5 µg/ml) in DMEM/F12 overnight at 37°C.
Overlapping ESTs were assembled manually.
Two final datasets were assembled.
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