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Th remaining cells were amplified overnight in 200 mL of SB media supplemented with 50μG.mL−1 of carbenicillin.
Finally, phages were amplified in a 50-ml baffled flask (2YT-Amp-Kan) overnight.
The generated recombinant viruses were amplified to >10 viral particles per ml.
To generate stable shRNA c-Yes clones, pSilencer-Yes shRNA or sh-control Ambion construct (srb1) were transfected into HT29 cells and neomycin-resistant colonies were amplified (400 µg/ml).
Transformed BL-21 cells were amplified by growing on LB broth medium supplemented with 50 µg/ml kanamycin.
Asx exons were amplified using genomic PCR.
The basic ones were amplified with 5'gcctgctcgaattcgggatg3'/3'gtctgcctgcatctagagga5'.
Positive clones were amplified and sequenced.
No products were amplified in water.
All cDNA samples were amplified in duplicates.
Plasmids were amplified in DH5α strains.
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