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This [F]SFB solution was passed over a preconditioned (10 mL sterile ethanol 96% and then 10 mL water for injection) Waters Sep-Pak C18 cartridge (Milford, MA, USA).
This fraction was collected in 30 mL water and passed over a preconditioned (10 mL sterile ethanol 96% and then 10 mL water for injection) Waters Sep-Pak tC18 (Milford, MA, USA).
The supernatant phase was removed and AgNPs were washed with 10 mL water for three times.
Each fabric was washed in 300 ml water for five minutes and then taken out to for squeezing.
Purified [18F]flumazenil was collected as the radiochemical peak eluting at 22 min, in a round bottom flask prefilled with 25 mL water for formulation.
The etching solution was composed of 5 mL acetic acid, 5 mL HCl, 6 g picric acid, 7 mL nitric acid, 100 mL ethanol, and 10 mL water for 1 2 s.
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The precipitate thus formed was washed with water several times and then redispersed in 8-mL water for characterization and further use.
In both situations, longer durations of TBC were observed during the swallow of 10 ml water (0.62 s for 3 ml water; 0.93 s for 10 ml water; and 0.56 s for solid).
The collected precipitate was dispersed in 450 mL water and sonicated for 2 h.
Briefly, cells were lysed with 0.5 ml water and sonicated for 30 seconds.
300 g dry algae were cut and soaked in 7000 mL water at rt for 24 h.
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