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At given time intervals, a certain volume of suspension (approximately 3 mL) was withdrawn.
A sample of 1 mL was withdrawn at different time intervals and the absorbance was measured at 427 nm.
Culture sample (5.0 ml) was withdrawn, and cells were centrifuged at 4,800 rpm for 5 min.
At the specified time interval, 2 mL was withdrawn from the receiver compartment and replaced by equal volume of fresh hydroethanolic solution and thus keeping a constant volume.
The sample (~0.2 mL) was withdrawn from the reaction vessel into a gastight® Hamilton™ syringe with a Mininert™ valve and immediately analyzed using a 4-point calibration curve.
Blood (~ 0.5 1 mL) was withdrawn by heart puncture, collected in a heparin-coated tube and centrifuged at 5000 RPM to separate plasma from blood cells.
Similar(16)
Biomass samples of 100 mL were withdrawn from the reactors three times per week.
Aliquots of 2 mL were withdrawn periodically and replaced with the same volume of fresh medium.
Aliquots (1 ml) were withdrawn every 2 3 days for cell count analysis and preserved in 3.7% formaldehyde.
After the reaction mixture was irradiated for a given time, the samples of 4 mL were withdrawn at each time and centrifuged for 20 min.
Samples of 2 mL were withdrawn each 6 h for the determination of reducing sugar (dinitrosalicylic acid method as cited before) and glucose concentrations (Glucose Liquicolor InVitro® kit).
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