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In the control beverage, rice starch (4.5 g/100 ml) was used instead of the fibre preparations.
Double-distilled water (100 ml) was used as solvent.
A volume of 14 ml was used in the cylinder for each measurement.
Homogenous algal suspension (5 ml) was used to inoculate each flask.
For inoculation, a final concentration of 1106 spores per mL was used.
A final volume of 1 ml was used.
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DKK1 protein (0.5 μg/ml) was used for stimulation.
The DNA crosslinking agent, mitomycin (0.5 μg/mL), was used as a positive control.
Therefore, the concentration of 10 μg/mL was used for in vitro studies on macrophages.
An increasing concentration of Trypan Blue from 1 to 3 mg/ml was used to quench the extracellular fluorescence.
E. coli LPS 055 B5 (0.1 ng/ml or ~1 EU/ml) was used as a positive control.
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