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The foregoing quinoline (0.5 g) and DBU (0.33 g, 320 µL, 2.1 mmol) in chloroform (25 mL) was treated with the amine (5 eq)., and the resulting mixture was stirred under reflux for 24 h.
A solution of 3,4-bis(benzyloxy phenol 3 (0.5 g, 1.63 mmol) in EtOH (6 mL) was treated with potassium hydroxide (91 mg, 1.63 mmol) and epichlorohydrin (151 mg, 1.63 mmol) and continued stirring at ambient room temperature for 6 h and then concentrated in vacuum.
The culture supernatant (800 mL) was treated with ammonium sulfate (70% saturation).
The solution 0.2 0.5 mL was treated as a GC-FID analytical procedure.
The appropriate commercial amine 3 7, 18 20 and 27 29 13.055 mmol) dissolved in DMF (20 ml) was treated with 3,5 dinitrobenzoyl chloride 2 (13.05 mmol).
The suspension containing the catalyst (1 g/100 ml) was treated in autoclave for 5 h at 160°C and autogenous pressure.
Once the bark (Fig. 1) extract (10 ml) was treated with 90 ml of silver nitrate solution, the color of the solution changed to dark brown color (Fig. 2) in <5 min.
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Approximately 1 mL of M. canis cells (5 × 10 CFU /ml) was treated with berberine hydrochloride, palmatine hydrochloride, their combination (0.5 mg/mL or 0.5 mg + 0.5 mg/mL) or clotrimazole (0.4 mg/mL) for 6,18, or 30 h.
Briefly, 0.5 mL serum (1 mg protein/mL) was treated with 0.5 mL 10 mM DNPH in 2 M HCl, or with 0.5 mL 2 M HCl alone for the blank.
SCF (4 μg/ml) was treated with protease inhibitors PMSF, EDTA, and DTT for 2 h and fibrinogenolytic activity of treated SCF was estimated post incubation.
The equal concentration of pristine MoS2 and MoS2 nanosheets after the liquid ultrasound exfoliation solution (10 mg/ml) was treated with ultrasound in ethanol for 30 min, respectively.
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