The antifungal potential of the test samples (2000 μg/mL) was screened by disc diffusion method (as described for the determination of antibacterial activity) against the fungal pathogens on Saborauds dextrose media.
As nanogold in a range of concentrations from 36 to 1000 ng/mL was screened for cytotoxic effects in different mammalian cell lines [ 14], we used AuNP concentrations ranging from 1.4 to 700 ng/mL that corresponds to 1.1 × 10 5.5 × 10 AuNPs/mL [ 17].
The synthetic array was screened at 1, 10 and 90 μg/mL of labeled protein and the pathogen array was screened at 90 μg/mL.
Participants received blood draws (3 mL) and were screened for depression at the beginning and end of the 12-week MAE program to detect changes in the outcome indicators.
All individuals within two clean 10 ml water suspensions were screened, counted with a stereomicroscope and fixed in 4% formaldehyde.
Among all tested transformants, two recombinant strains, labeled as P. pastoris GSAu4-2 and GSAEM4-8, expressing the highest reAuXyn11A and reAEXynM activities of 646.6 and 580.8 U mL-1, respectively, were screened.
The transformed cells were screened in LB medium containing kanamycin (100 μg/ml) at 37°C overnight.
After 48 h of continuous culture, cells were screened using G418 (400 μg/mL).
Independent clones resistant to Blasticidin (10 µg/ml) were screened for acap-B gene disruption by Southern hybridization.
Following transfection, clones were selected in G418 (2 mg/mL, invitrogen), and selected clones were screened for targeted integration via PCR.
In order to identify HAsik1 or p57 overexpressing ES cells, two weeks after selection with 1.2 µg/mL puromycin, the resistant colonies were screened by RT-PCR using the primers sik1UP and sik1LW for HAsik1 clones and with vector up and p57low for the p57 clones.
