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The culture medium (0.5 mL) was sampled per day and centrifuged at 4°C, 10,000 rpm for 5 min. Cell pellet and supernatant were separated.
The reaction solution (10 mL) was sampled from the three photocatalyst preparations at 0, 1 and 7 h, then filtered through a 0.22-μm membrane to remove the fungal cells.
Blood (5 ml) was sampled in anticoagulant-free tubes and kept at room temperature for 1 h before the serum was isolated (centrifugation at 2000 g for 10 min at 20 °C).
Total bone marrow (around 5 ml) was sampled after the last boost to isolate RNA using Tri Reagent (Molecular Research Center Inc., Cincinnati, USA) according to the manufacturer's instructions.
Blood (3 mL) was sampled from every patient.
For milk, 10.0 ml was sampled and its pH was lowered to 4.6 or below.
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Aliquots of 0.2 mL were sampled from the beaker at predetermine intervals.
Aliquots of 1.5 mL were sampled over 160 min at 20-min intervals.
Suspension aliquots of 10 ml were sampled at 2, 4, 22 and 24 h incubation, respectively, and immediately analyzed on the continuous flow analyzer to determine their nitrate concentrations.
Per sampling time point, approximately 5 ml blood was sampled.
At given time intervals, about 3 mL solution suspension was sampled and immediately centrifuged.
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