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From the sample, 10 mL was removed.
A total of 1.2 mL was removed, from each tube, over the course of the experiment.
The release medium of 4 mL was removed and replaced by fresh buffer solution at regular time intervals.
After another very brief vortexing, ca. 0.2 mL was removed for the evaluations of germination and growth on selective medium (see below).
From the stock culture 0.2 ml was removed and dispersed in 20 ml of sterile phosphate buffer (50 mM, pH 7.0); vortex well.
The supernatant (4.0 mL) was removed and immediately replaced by the same volume of background solution (0.01 M NaNO3), and the vials were resealed and shaken.
Similar(16)
Samples (1.5 ml) were removed every 24 h and the presence of BLV proteins was determined using an indirect ELISA assay in which the antigen reaction with the monoclonal antibodies was evidenced by peroxidase labeled anti-mouse immunoglobulins.
Following the initial sampling, culture aliquots of 30 mL were removed at intermittent time points for the same purpose.
Thereafter, the 12 ml were removed and slides were gently rinsed twice with 12 ml of PPB to remove loosely attached cells.
At selected time intervals, aliquots (0.5 mL) were removed from the mixture solution, replacing by an equal volume (0.5 mL) of fresh SBF.
Samples of 2 ml were removed from the liquid cultivations at intervals and mycelium was separated from the supernatant by centrifugation.
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