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Vasconcellos et al. proposed an analytic change for improving performance with respect to bias and mean-squared error, and the bias in the estimation of the roughness parameter of the ( {mathcal{G}}_I^0 ) distribution by maximum likelihood (ML) was quantified [5].
The number of MAP2+BrdU+ cell in the ML was quantified in (D′ ).
The percentage of GABARα6+NeuN+/NeuN+ cells in the ML was quantified in (D′′ ).
The total number of NeuN+ cells in the ML was quantified in (H′ ).
For GCs, dendritic spine density in the outer one-third of the dorsal ML was quantified.
(C′ ) The number of NeuN+ cells in the ML was quantified and compared.
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(E ) Sections of adult control and Nf1 hGFAPCKO cerebella were stained for NeuN/GABA and the number of GABA+ GABAergic neurons in the ML were quantified in (E′ ).
Adhesion of endothelial cells to polystyrene microtitre plates coated with perlecan (20 nM, 9.4 μg mL-1) or fibronectin (20 nM, 10 μg mL-1) was quantified after 2 h incubation with HCAECs (2.5 × 10 cells mL-1, 50 μL) at 37 °C by staining with crystal violet as described previously [5].
Binding of [I]-labeled FGF-2 (50 μl; ca. 120 000 cpm well-1) to surface-absorbed perlecan (32 nM, 15 μg mL-1) was quantified by gamma counting as described previously [5].
The distribution of Tbr2+BrdU+ cells (arrows) in the EGL-ML-IGL was quantified 24 hr and 48 hr after BrdU-pulse in (F′ ) and (G′ ), respectively.
The viral titers (infectious units per ml, iu ml−1) were quantified by infecting BHK-21 cells with serial dilutions of viral stock and analyzing EGFP or DS-Red expression via fluorescence microscopy on a Leica DM IL microscope (Leica Microsystems Wetzlar GmbH, Germany).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com