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Tumor cells suspension (2.5 × 106 per ml) was prepared in saline.
Each solvent mixture (5 mL) was prepared in 10 mL vials.
Starch solution (1% w/v, 50 mL) was prepared using Tris-HCl buffer (100 mM, pH 8.0).
Similar(57)
MTT stock (5 mg/mL) was prepared earlier in 100 mL PBS.
A concentration of 10,000 nematodes/ml was prepared for each nematode.
A stock solution of sodium glutamate (200 μg/ml) was prepared in water.
An internal standard solution (3.58 mg/mL) was prepared by resolving styralyl propionate in MTBE.
A standard stock solution of brimonidine tartrate (1 mg/mL) was prepared using the mobile phase.
MTT stock solution 5 mg/ml was prepared in distilled water.
A suspension of 0.5 mg/ml was prepared and sonicated for 1 h before the analysis.
First, fresh Fmoc-FF stock solution (3 mg/mL) was prepared by the above method.
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