Exact(3)
The central gel compartment of 2 mL was packed with dye ligand affinity adsorbent.
Q-Sepharose Fast Flow (0.5 ml) was packed in plastic filtration tubes (Supelco) and washed with 10 ml of purified water.
The serum was collected, filtered to remove bacteria, and 1.5 ml was packed in an EP tube, which was stored at -70°C in liquid nitrogen.
Similar(57)
The supports (20 mL) were packed into a column (XK16/20, Pharmacia LKB Biotechnology, Uppsala, Sweden; 16 mm i.d. × 100 mm length).
Water samples (500 ml) were packed in glass bottles and sealed.
A 400 ml glass column was packed with 60 g of fine silica gel (1.10757.1000).
For chromatographic purification a 5 ml plastic pipette was packed with Sepharose CL-4B (Pharmacia) equilibrated in microinjection buffer (5 mM Tris/HCl, pH 7.4, 0.1 mM EDTA, 5 mM NaCl) (Zeilhofer et al. 2005), the linearized BAC 11-DNA was mixed with BAC 11-DNAl blue and chromatographed.
For cleanup, a 6-mL solid phase extraction column was packed with a filter paper, 1.0 mL 44% sulfuric acid impregnated silica, 1.0 mL activated silica, 1.0 mL 10% silver nitrate impregnated silica, and a wad of glass wool on top to prevent dusting.
Briefly, 1 ml of drug-loaded nanoparticles was packed in a dialysis membrane (Spectra/Por 6, MWCO 3000, Spectrum Laboratories, Inc., TX, USA) and both the ends were sealed.
A 100-mL, dry three-necked bottle was packed in an ice bath and 1.97 g (25 mmol) Se and 1.13 g (30 mmol) NaBH4 were added under a nitrogen atmosphere.
After resuspension, the semen was packed into 0.5 ml polyvinyl chloride straws (IMV International, St Paul, MN, USA).
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