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Normal saline (60 mL) was instilled separately into the right middle lobe (non-Tween-injured tissue) and the right dorso-caudal lobe (Tween-injured tissue) to collect BALF.
Sterile isotonic saline (100 ml) was instilled in 20-ml portions.
Saline (100-200 mL) was instilled into the rectum or a rectal balloon was used to stabilize the prostate position.
Saline (10 ml) was instilled into the tube, and the drainage fluid was collected on the other side.
To produce acute superficial injury in the fundic mucosa, absolute ethanol (1 ml) was instilled into the stomach by gastric intubation.
Sterile normal saline (0.5 ml) was instilled into the endotracheal tube and 3 4 manual breaths were provided using a self inflating bag.
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The first stopcock is turned on to the patient, and the normal saline (20 ml) is instilled into the bladder through the urinary catheter [11].
For the BAL, three aliquots of normal saline (1 ml/kg, maximum 40 ml) were instilled in the right middle lobe through the bronchoscope and the fluid retrieved by mechanical suction.
The first stopcock is turned on to the patient, and the normal saline (20 ml) is instilled into the bladder through the urinary catheter [ 11].
As a control, normal saline (2 ml kg−1) was instilled in rats.
HCl (0.1 N, 2 ml kg−1) was instilled into male Sprague Dawley rats that were pretreated with or without JTE-607 (30 or 75 mg kg−1 h−1).
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