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3-Ethoxy-2,4-dioctylcyclobut-2-enone (11): Using a 10 mL injection loop, a solution of 1-ethoxydec-1-yne (3a; 0.364 g, 2.0 mmol) in toluene (8 mL) was inserted into a continuous flow of toluene (1 mL/min), which then passed through a stainless steel tube (10 mL; 1 mm i.d).
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Sample C and D have the same structure with that of sample A and B, respectively, except that an ultra-thin InAs layer with a thickness of 1 ML is inserted at one of the interfaces of each GaAs/Al 0.3 Ga 0.7As QWs, i.e., forming Al 0.3 Ga 0.7As/InAs/GaAs/Al 0.3 Ga 0.7As structures.
Then a 33-gauge needle equipped with 1 ml syringe was inserted into the mammary duct of L4 or R4 and 0.1 ml S.
A 0.3-cm3 syringe (29G needle) containing 5 × 104 4T1lucellsls in 0.1 ml DPBS was inserted slowly into the chest in the second intercostal space directly into the left ventricle.
DUI was performed using a specially designed flexible device (length 180 cm, outer diameter 4 mm, working channel 1.8 mm, working channel's volume 1.5 ml) that was inserted through an artificial insemination spirette to cross the cervix lumen and moved into one uterine horn as far as possible.
An incision was made in the trachea and a sterile-flexible cannula attached to a 3 mL syringe was inserted.
At serial time points a tracheotomy was performed after mice were anesthetized with a mixture of ketamine-xylazine; a sterile-flexible cannula attached to a 3 mL syringe was inserted into the trachea.
On the same occasion, an 18 gauge needle connected to a 10 ml syringe was inserted into the periumblical fat tissue.
After sterile draping and sufficient disinfection, a 22-gauge needle attached to a 10-ml syringe was inserted into and withdrawn from the lymph node three times under aspiration while sonographically monitored.
A half-cut 4 mL glass vial was inserted to one end as a cap.
Finally, a syringe needle with 1 mL of PBS was inserted three times into the nasopharynx to collect the nasal wash.
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