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The concentrate (approximately 200 mL) was diluted in 5 L.
Enzyme solution (1 ml) was diluted with phosphate buffer (pH 7) and mixed with casein (1%).
The supernatant (1 ml) was diluted properly with ethanol aqueous solution (70%, V/V, pH 2).
Before the analysis, each juice sample (50 mL) was diluted in different proportions using distilled water.
A solution of pseudodiosgenin diacetate (4) (15.4 g, 30.8 mmol) in acetic anhydride (76 ml) was diluted with water (4.8 ml) and acetic acid (50 ml).
The enzyme (0.2 ml) was diluted into 20 ml of water and then sprayed onto 1 kg of total mixed ration (dried in an oven at 55 °C and ground through a 4.5-mm screen) prior to feeding.
Similar(32)
Cisplatin (Bedford Laboratories, Bedford, OH) was administered intraperitoneally. 1 ml of cisplatin stock (1 mg/ml) was diluted up in 10 ml of 0.9% saline.
Briefly, 0.5 mL of the plant extract (1 mg/mL) was diluted with 2 mL of distilled water.
One milliliter of this culture (approximate density 2 x 10 cells/ml) was diluted with 9 ml of fresh Luria broth medium.
Briefly, captured antibody 2 μg/mL was diluted in 10 mL of PBS 1X, 100 μL was added to each well in 96 well Maxisorb microplate (Nunc, Denmark), and was incubated overnight at 4°C.
50 µl of 2 µm carboxyl-terminated polystyrene beads from the stock solution (∼2×1010 beads/ml) was diluted to a final volume of 1 ml in Milli-Q water.
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