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A 2 mL sample of head-space gas (total, approximately 29.5 mL) was applied to a gas chromatograph (ODSA-P2, Alpha M.O.S. Japan).
Collagen solution (2 ml) was applied on the silicon surface (Silicon Valley Microelectronics, CA) and air dried in a fume hood overnight.
A 600 μL sample of head-space gas (total, approximately 14.5 mL) was applied to a gas chromatograph equipped with a flame photometric detector and a data processor C-R8A Chromatopac (Shimadzu).
A 1 mL sample of head-space gas (total, approximately 14.5 mL) was applied to a gas chromatograph equipped with a flame photometric detector and a data processor C-R8A Chromatopac (Shimadzu).
The spore suspension (1 ml) was applied by using a sterile sprayer.
The injection (4 mL) was applied once every other day or once a day, over a course of 10 days.
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During the surgery, a blue dye (2 ml, 50 mg/ml) was applied at the lesion site by a subdermal injection.
Seven ml of larval hemolymph (2.91 × 108 pfu/ml) was applied to Sephacryl S-1000 SF column equilibrated with PBS.
2 mL of AGE-BSA solution with a concentration of 53.8 mg/mL was applied in each purification process, using a flow rate of 1 mL/min.
Mitomycin C (0.2 mg/ml) was applied on the bare sclera for 1 min before preparation of the scleral flap.
Mitomycin C (0.2 mg/ml) was applied to the bare sclera for 1 min before preparation of the scleral flap.
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