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The volume of evaporated extract was adjusted to 10 ml using the same solvent.
In this study, DO measurements were carried out in sample volumes ranging from 2 mL to 10 mL using the PM.
The sample was then clarified at 13,000 g for 10 min and the supernatant was brought to 50 ml using the above pellet-dissolving phosphate buffer.
For analysis, an appropriate aliquot from the prepared sample solutions, spiked with 300 µL TNX stock solution, was diluted to 10 mL using the same solvent.
The predicted left liver graft volume with the middle hepatic vein was found to be 403 mL using the region-growing method with 3D CT software.
After each pickling treatment, the pickling solution volume was restored to 30 mL using the same vinegar used for pickled soybean production.
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Titers were calculated as 50%% tissue culture infectious doses (TCID50)/ml, using the Reed and Muench method [ 65] to estimate endpoints.
Total RNA was extracted from 2 mL of cells at 2 3 × 109 cells/ml, using the RNeasy kit (Qiagen, Basel, Switzerland), as previously described (15 16).
Total RNA was extracted from 2 mL of cells at 2 3 × 109 cells/ml, using the RNeasy kit (Qiagen, Basel, Switzerland), as previously described [ 32, 33].
All free model parameters were estimated by RA×ML using the GAMMA+P-Invar model of rate heterogeneity with an ML estimate of the alpha-parameter.
The minimum detectable concentration of THC in blood was 10 ng/ml using the ELISA screening test and 1 ng/ml using the LC/MS/MS confirmation test.
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