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The fraction was concentrated to 10 mL using a rotary evaporator at 40 °C.
For measurements, sample (50 mg) was dissolved with nitrohydrochloric acid (5 ml) using a microwave oven.
To evaluate short- and long-term vocal outcomes after medialization laryngoplasty (ML) using a silastic implant in patients with unilateral vocal fold paralysis (UVFP).
The extracts were concentrated to about 2 mL using a rotary evaporator operating at temperature of 30 C at a reduced pressure.
Batches of 10 fourth-stage larvae of C. pipiens pallens were separately put into Beakers (100 mL) containing each test solution (40 mL) using a pipet.
Injection solutions of the BPA-fructose complex (BPA-fr) were prepared at a concentration of 10 mg BPA/0.43 mL using a previously published procedure with modifications [6].
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The amount of cyanomethaemoglobin formed was detected at 540 nm using a spectrophotometer and converted to mg/mL using a standard curve of rat Hb (Sigma).
The standard curve range for rapamycin is from 0.250 to 50.0 ng/mL, using a whole blood sample volume of 0.200 mL.
The spores' concentration was adjusted to 5 × 105 cfu/ml using a hamacytometer.
The cells were counted (1 × 108 cfu/mL) using a UV spectrophotometer.
The fungal spores concentrations were adjusted to 5 × 105 spores/mL using a haemocytometer before each test.
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