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The fraction was concentrated to 10 mL using a rotary evaporator at 40 °C.

For measurements, sample (50 mg) was dissolved with nitrohydrochloric acid (5 ml) using a microwave oven.

To evaluate short- and long-term vocal outcomes after medialization laryngoplasty (ML) using a silastic implant in patients with unilateral vocal fold paralysis (UVFP).

Batches of 10 fourth-stage larvae of C. pipiens pallens were separately put into Beakers (100 mL) containing each test solution (40 mL) using a pipet.

The fraction was concentrated to 10 mL using a rotary evaporator at 40 °C, divided into five 2-mL portions, and freeze-dried.

The solution was evaporated with toluene (2 × 20 ml) and chloroform (2 × 20 ml) using a rotary evaporator, precipitated in acetonitrile, and dried under vacuum.

The extracts were concentrated to about 2 mL using a rotary evaporator operating at temperature of 30 C at a reduced pressure.

Injection solutions of the BPA-fructose complex (BPA-fr) were prepared at a concentration of 10 mg BPA/0.43 mL using a previously published procedure with modifications [6].

The supernatant was evaporated to approximately 1 mL using a rotary evaporator set at 55°C before being dried to completeness in a heating block set at 55°C overnight.

SLNB: At the day before operation, sentinel lymphoscintigraphy was performed, with the injection of 99 m Tc (0.2 ml) using a Techne Phytate Kit.

The upper organic layer was removed and filtered through sodium sulphate and concentrated to 5 ml using a rotary evaporator set at 40 °C.

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