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Saturated aqueous K2CO3 was added (5 mL) to destroy any unreacted phosphorus pentoxide.
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Then, n-hexane (30 mL) was added to destroy the one-phase solution and form a two-phase mixture, so the hydrophobic colloidal NaYF4:20% Yb3+, 2% Er3+ UCNPs were extracted into the upper layer (n-hexane region).
Na2SO3 10% in water (0.055 mL) was added to destroy the excess of peroxide.
The reaction was kept at −78°C for 15 min, then warmed to room temperature and stirred for a further 30 min. A solution of Na2SO310%% in water (1.2 mL) was added to destroy the excess of peroxide.
After stirring was continued for 12 h, 10 ml of acetone was added to destroy the microemulsion.
After n-hexadecanol was completely gone, the reaction was quenched at 0°C by stirring with the saturated NaHCO3 solution (40 mL) containing Na2S2O3 (3 g) for 10 min to destroy any unreacted Dess-Martin reagent.
Then 1 ml of each liposomal suspension in PBS was suspended in 10 ml chloroform, followed by vigorously vortex-mixing for 10 min, to destroy the liposome structure, releasing the drug into the organic phase.
To destroy the surplus of nitrite, 0.5 mL of amidosulfonic acid (50 g/L) was added.
For further investigation of light-diffracting structures inside the cells, a 1-ml culture was exposed to high pressure (150 bar) to destroy possible gas vesicles.
Except fermented milk, 10 mL of each raw milk sample was heated at 80 °C for 10 min to destroy natural inhibitors lysozyme and lactoferrin (Hillerton et al. 1999).
During sample preparation, 200 μL of whole blood was chemically digested with a 2-mL mixture of nitric acid and water peroxide (1 1) and heated at 120°C to destroy the blood matrix and liberate the metals.
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