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Hybrids were fed living Artemia larvae and were maintained individually in 500 ml tanks or in groups of approximately 10 in 3 L tanks.
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In addition, 300 mL tank sea water (W) sample was collected and filtered by 0.22 μm pore size hydrophilic polyethersulfone membrane filter.
Within a 50-mL tank, the electrolytic solution as described above received a voltage variation of 160 V initial tension at zero time and a final tension at the preset end-time for each group of samples.
The reaction was carried out in a 500 ml Stirred tank reactor using heptane and hexane as solvents.
Batch fermentation was performed at 30 °C, in a 50 mL stirred tank bioreactor with controlled heating and stirring, built in the laboratory of UNL (Argentina).
The kinetic experiments were carried out in a 100 mL stirred tank reactor at various temperatures (30, 35, 40 and 45 °C) at 200 rpm.
Gas samples were taken out from the autoclave in a 100 ml gas tank and injected with a gas syringe on a gas chromatography (Agilent, 7890A) equipped with a thermal conductivity detector (TCD), split/splitless injection system and Porapak Q column.
One ml of tank seawater was collected at inoculation time (t = 0 h), 1 h post inoculation (t = 1 h) and then at 12 h intervals (t = 12 h, t = 24 h, t = 36 h) from four infection tanks.
100 μL was added to a vial containing embryos in 6 ml of tank water.
Batch fermentations were developed at 28°C in a 500-mL stirred tank bioreactor, equipped with controlled heating and stirring systems.
The poly(NVF-co-MOENVF) (0.20 g, solid) and NaH (0.4 mmol, 0.011 g, solid) were combined in a 50-mL glass tank.
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