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For this assay, the media was prepared in the same manner as above, but the two substrates were then moved to a large (150 ml) square petri plate.
The two media were carefully removed from the small petri dish lids and placed in the bottom half of a 150 ml square petri dish.
Bioconversions were performed in 96-sdMTP (Waters, 2 ml square collection plate) for 3 hours at 37°C with shaking essentially as described [ 26].
Cultures were grown for 4 hours in 96-sdMTP (Waters, 2 ml square collection plate) at 30°C in a Titramax 1000 shaker (Heidolph) at 1050 rpm, 1.5 mm shaking diameter.
The microplate was a standard storage plate (1.2 mL square round-bottom plate, ABGene Ltd ,Epsom, UK) with 800 µL working volume per well located on a magnetic stirrer (710CI, V&P Scientific) and mounted on the deck of a liquid handling robot (Evo 150, Tecan UK Ltd, Reading, UK).
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FAMEs synthesis reactions were carried out in 100 ml squared bottles for 24 h at 35°C with 250 rpm agitation in a horizontal shaker.
The Pearson chi-square, ML chi-square, paired t test, factor analysis and structure equation modeling (SEM) were used to get the statistical results.
The Pearson Chi-square tests and ML Chi-square were performed and recorded on Table 3.
A multi-well centrifuge was used (Centrifuge 5810R; Eppendorf, Hamburg, Germany, equipped with a 2 mL deep square well plate format (Abgene, Epsom, UK)) using a method previously described by Tait et al. (2009).
Overall statistical significance of differences among syndromes is based on ML chi-square test Overall statistical significance of differences among syndromes is based on ML chi-square test.
In section 4, we address the problem of localization by combining DoA estimates of multiple sensors: we first derive the CRB of the achievable localization error, highlighting its dependence on distances and angles between sensors and target, and then we introduce practical algorithms: maximum-likelihood (ML), least-squares (LS), and Stansfield estimators.
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