The minimum sample volume is 2 mL per time point.
Samplings of 1 mL per time point were collected at 1, 5, 10, 15, 20, 30, 45, 60, 120, and 180 minutes, and the olanzapine pamoate concentration in each sample was analyzed using liquid chromatography-mass spectrometry.
The best way to evaluate GFR is given by the calculated creatinine clearance using the formula UV/P (ml/min) (U being the urinary creatinine concentration in µmol/l, V the urinary volume expressed in ml per unit time, P the Scr concentration in µmol/l).
Peripheral blood samples (6 ml per sampling time) were collected in heparinised tubes and centrifuged at 1000 g for 15 min at 4°C.
The skeletally immature patients had been under medical treatment of hypophosphatemic rickets, which included anhydrous phosphate (Joulie's) oral solution (average intake of 15 mL per dose, five times daily) along with vitamin D or its metabolites.
Then, they were decocted with water (2500 mL, 1500 mL, and 1500 mL) for 3 times, 30 mins per time, respectively.
The cell concentration was adjusted to 105 cells/ml, 1 mL samples of each strain were placed in 1.5 mL Eppendorf tubes (4 samples per time point) and subjected either to the background level of radiation or to a radiation field of 0.05 mGy/hr created by 188Re/188W isotope generator for up to 30 hr at 30°C.
Growth curves of Chlorella sp. and Scenedesmus sp. in terms of CC (million cells per ml) vs time are shown in Fig. 1.
Blood flow in the hepatic artery, renal artery, celiac trunk, and portal vein was continuously measured in all animals throughout the experiments by means of ultrasonic transit time flowmetry (mL per minute) and an HT 206 flow meter (Transonic Systems Inc).
These points were then used in the following equation: (3) μ max 1 h = Δ lnBDW ΔT = ln BD W t 2 - BD W t 1 T 2 - T 1 where BDW was measured in grams per 1,000 mL and time in hours.
Then filters were TCA (Trichloroacetic Acid) precipitated first at least 3 hr in two batches of cold 5% TCA, 2% PPNA solution (PPNA : tetrasodium diphosphate) (20 mL per filter) and three times two hours in cold 5% TCA then washed twice in ethanol 95%, dried and counting of the incorporated TCA precipitable radioactivity was done in 5 mL of Liquifluor (Amersham).
