Sentence examples for ml per slide from inspiring English sources

The volume of blocking solution should be at least 20 ml per slide.

Finally, the glands were cleared by submersion in xylene, approximately 100 ml per slide, which was changed daily until the epithelial structures could be clearly observed.

Cells were then transferred to a tube, pelleted, suspended in 2.5 ml of DPBS, 0.5% BSA, 10 m NaN3, and cytocentrifuge slides were prepared, with a StatSpin Cytofuge 2 (Norwood, MA, USA), using 0.2 ml per slide.

The slides were immersed in pre-chilled lysis solution from the kit (approximately 5 ml per slide) and incubated at 4 °C for 1 h before being transferred to an alkaline solution pH >13 (0.6 g NaOH pellets, 200 m EDTA in 50 ml ddH2O) for 1 h at room temperature in the dark.

A total of 40 mL of BM was aspirated from anterior and posterior iliac crests bilaterally (10 mL per site) and after separation by density centrifugation, mononuclear cells (MNC) were collected and cytospins were prepared (5 × 105 MNC/slide).

After flicking the cells into medium, an aliquot of 0.5 ml was used for differential cell count (typically 2×104 cells per slide) and the remainder (4.5 ml) was processed immediately for RNA extraction.

On the day of transfection cells were seeded at 3.5 × 106 (HEK293T, HEK293, HEK293-LBD, PC-3, Cos7, HepG2 and WI-38) or 1 × 106 (HeLa) cells per slide in 8 ml complete media in quadriperm (VivasCos7ce) disHepG2

Cells were collected into centrifuge tubes and spun at high speed for 10 min. Cell pellets were resuspended to 1×103 cells per ml and cytospins were prepared with 100 µl of cells per slide.

A total of 20 000 cells per ml in PBS were mixed with low melting agarose at a 1 : 10 ratio and 900 cells were added per slide to allow to solidify.

Then, 1 1.2 mL of incubation buffer (50 mM Tris-HCl, 5 mM KCl, 2 mM CaCl2, 2 mM MgCl2, 120 mM NaCl, pH 7.4 °C °C) was applied per slide, and the slides were pre-incubated for 15 min at room temperature.

A total of 100 randomly selected cells from two replicate slides (50 cells per slide) were examined per sample.